Ingeniería de bacterias para aplicaciones biomédicas

Investigador Principal: LUIS A. FERNÁNDEZ HERRERO

Ruano-Gallego D., Álvarez B., and L.A. Fernández (2015) "Engineering the controlled assembly of filamentous injectisomes in E. coli K-12 for protein translocation into mammalian cells." ACS Synthetic Biology (in press). PMID: 26017572 DOI: 10.1021/acssynbio.5b00080

Piñero-Lambea C., Ruano-Gallego D., and L.A. Fernández (2015) "Engineered bacteria as therapeutic agents" Current Opinion in Biotechnology 35: 94-102. PMID: 26070111 DOI: 10.1016/j.copbio.2015.05.004

Piñero-Lambea C., Bodelón G., Fernández-Periáñez R., Cuesta A.M., Álvarez-Vallina L., and L.A. Fernández (2015) "Programming controlled adhesion of E. coli to target surfaces, cells and tumors with synthetic adhesins" ACS Synthetic Biology 4:463-473. PMID: 25045780 DOI: 10.1021/sb500252a

Bodelón G., Marín E., and L.A. Fernández (2015) "Analyzing the role of periplasmic folding factors in the biogenesis of OMPs and members of the type V secretion system" Methods in Molecular Biology 1329: 77-110. PMID: 26427678 DOI: 10.1007/978-1-4939-2871-2_7

Campuzano S., Salema V., Moreno M., Gamella M., Yañez-Sedeño P., Fernández L.A.*, and J. M. Pingarrón* (2014) "Disposable amperometric magnetoimmunosensors using nanobodies as biorecognition element. Determination of Fibrinogen in plasma" Biosensors and Bioelectronics 52:255-260. PMID: 24060974 DOI: 10.1016/j.bios.2013.08.055

Salema V., Marín E., Martínez-Arteaga R., Ruano-Gallego D., Margolles Y., Teira X., Gutierrez C., Bodelón G., and L.A. Fernández* (2013) "Selection of single domain antibodies from immune libraries displayed on the surface of E. coli cells with two ?-domains of opposite topologies" PLoS ONE 8 (9):e75126 PMID: 24086454 DOI: 10.1371/journal.pone.0075126

Salema V., and L.A. Fernández* (2013) "High yield purification of nanobodies from the periplasm of E. coli as fusions with the maltose binding protein" Protein Expression and Purification 91:42-48. PMID:23856605 DOI: 10.1016/j.pep.2013.07.001

Van Audenhove I., Van Impe K., Ruano-Gallego D., De Clercq S., De Muynck K., Hubert T., Perdu S., Vanloo B., Verstraete H., Fernández L.A., and J. Gettemans (2013) "Mapping cytoskeletal protein function in cells by means of nanobodies" Cytoskeleton (in press). PMID: 23818458 DOI: 10.1002/cm.21122

Bodelón G., Palomino C., and L.A. Fernández (2012) "Immunoglobulin domains in E. coli and other enterobacteria: from pathogenesis to applications in antibody technologies" FEMS Microbiology Reviews 37:204-250 PMID:22724448 DOI: 10.1111/j.1574-6976.2012.00347.x

Jurado P., Fernández L.A., and V. de Lorenzo (2012) "Production and characterization of a recombinant single-chain antibody (scFv) for tracing the sigma 54 factor of Pseudomonas putida" Journal of Biotechnology 160:33-41. PMID:22206981

Fernández L.A., and Serge Muyldermans (2011) "Recent developments in engineering and delivery of protein and antibody therapeutics" Current Opinion in Biotechnology 22:1-4. PUBMED: 21862312

Palomino C., Marín E., and L.A. Fernández (2011) "The fimbrial usher FimD follows the SurA-BamB pathway for its assembly in the outer membrane of Escherichia coli" Journal of Bacteriology 193:5222-5230. PUBMED: 21784935

Blanco-Toribio A., Muyldermans S., Frankel G, and L.A. Fernández (2010) "Direct injection of single-domain antibodies from E. coli into human cells" PLoS ONE 5(12): e15227. PUBMED: 21170340

Marín E., Bodelón G., and L.A. Fernández (2010) "A comparative analysis of the biochemical and functional properties of C-terminal domains of autotransporters" Journal of Bacteriology 192: 5588-5602. PUBMED: 20802036

Bodelón G., Marín E., and L.A. Fernández (2009) “Role of periplasmic chaperones and BamA (YaeT/Omp85) for folding and secretion of Intimin from enteropathogenic E. coli strains” Journal of Bacteriology 191:5169-5179.[PUBMED]

Munera D., Palomino C., and L.A. Fernández (2008) “Specific residues in the N-terminal domain of FimH stimulate type 1 fimbriae assembly in Escherichia coli following the initial binding of the adhesin to FimD usher ”Molecular Microbiology 69: 911-925.[PUBMED]

Demuth, A., Aharonowitz, Y., Bachmann, T. T., Blum-Oehler, G.Buchrieser, C., Covacci, A., Dobrindt, U., Emody, L., van der Ende, A., Ewbank, J., Fernández, L. A., Frosch, M., Portillo, F. G., Gilmore, M. S., Glaser, P., Goebel, W., Hasnain, S. E., Heesemann, J., Islam, K., Korhonen, T., Maiden, M., Meyer, T. F., Montecucco, C., Oswald, E., Parkhill, J., Pucciarelli, M. G., Ron, E., Svanborg, C., Uhlin, B. E., Wai, S. N., Wehland, J., Hacker, J. (2008) “Pathogenomics: an updated European Research Agenda" Infection, Genetics and Evolution 8: 386-393.[PUBMED]

Munera D., Hultgren S., and L.A. Fernández. (2007) “Recognition of the N-terminal lectin domain of FimH by the usher FimD is required for type 1 pilus biogenesis” Molecular Microbiology 64: 333-346. [PUBMED]

Garcillán-Barcia M.P., Jurado P., González-Perez B., Moncalián G., L.A. Fernández and F. de la Cruz (2007) “Conjugative transfer can be inhibited by blocking relaxase activity within the recipient cell with intrabodies” Molecular Microbiology 63: 404-416. [PUBMED]

Jurado P., de Lorenzo V. and L.A. Fernández (2006) “Thioredoxin fusions increase folding of single chain Fv antibodies in the cytoplasm of E. coli: evidence that chaperone activity is the prime effect of thioredoxin” Journal of Molecular Biology 357: 49-61. [PUBMED]

Jurado P., L.A. Fernández and V. de Lorenzo (2006) “In vivo drafting of single-chain antibodies for regulatory duty on the sigma 54-promoter Pu of the TOL plasmid ” Molecular Microbiology 60: 1218-1227. [PUBMED]

L.A. Fernández (2005) “Exploring Prokaryotic diversity: there are other molecular worlds” Molecular Microbiology 55: 5-15. [PUBMED]

Fraile S., Muñoz A., .de Lorenzo V., and L.A. Fernández (2004) “Secretion of proteins with dimerization capacity by the hemolysin type-I transport system of Escherichia coli” Molecular Microbiology 53: 1109-1121. [PUBMED]

L.A. Fernández (2004) “Prokaryotic expression of antibodies and affibodies” Current Opinion in Biotechnology 15:364-373. [PUBMED]

Veiga E.,.de Lorenzo V., and L.A. Fernández (2004) “Structural tolerance of bacterial autotransporters for folded passenger protein domains” Molecular Microbiology 52: 1069-1080. [PUBMED]

Veiga E.,.de Lorenzo V., and L.A. Fernández (2003) “Neutralization of enteric coronavirus with Escherichia coli cells expressing scFv-autotransporter fusions” Journal of Virology 77:13396-13398. [PUBMED]

Veiga E.,.de Lorenzo V., and L.A. Fernández (2003) “Autotransporters as scaffolds for novel bacterial adhesins: surface properties of Escherichia coli cells displaying Jun/Fos dimerization domains” Journal of Bacteriology 185: 5585-5590. [PUBMED]

Jurado P., L.A. Fernández, and V. de Lorenzo (2003) “Sigma 54 levels and physiological control of the Pseudomonas putida Pu promoter” Journal of Bacteriology 185: 3379-3383. [PUBMED]

Jurado P., Ritz D., Beckwith J., de Lorenzo V., and L.A. Fernández (2002) “Production of functional single-chain Fv antibodies in the cytoplasm of Escherichia coli” Journal of Molecular Biology 320: 1-10. [PUBMED]

Veiga E., Sugawara E., Nikaido H., de Lorenzo V., and L.A. Fernández (2002) “Export of autotransported proteins proceeds through an oligomeric ring shaped by C-terminal domains” EMBO Journal 21: 2122-2131. [PUBMED]